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nis elements imaging software  (Nikon)


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    Structured Review

    Nikon nis elements imaging software
    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Nis Elements Imaging Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nis+elements+imaging+software/NIS-Elements/pmc12811640-82-5-10
    Average 99 stars, based on 39515 article reviews
    nis elements imaging software - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software

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    Imaging:

    Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters
    Article Snippet: Live-cell imaging was conducted using a Nikon Ti-LAPP H-TIRF module with a CFI Apochromat TIRF 60 × oil objective (NA 1.49) or Apochromat TIRF 100 × oil objective (NA 1.49). .. Image acquisition was controlled by NIS-Elements Imaging Software (Nikon), with frames captured every ∼ 2 min. ..

    Article Title: PCB Exposure in Adult Male Mice Reduces Proliferating Cells in the Prostate but Minimally Alters Voiding
    Article Snippet: .. An Eclipse Ci compound microscope fitted with a DS Ri2 camera (Nikon Instruments Inc., New York, NY, USA) with NIS elements imaging software (D 5.02.01) (Nikon Instruments Inc., New York, NY, USA) was used for imaging. ..

    Article Title: Single molecule peptide sequencing
    Article Snippet: .. The Sutter Lambda 10-3 lter wheels (Sutter Instrument, CA, USA), motorized stage (Prior Scientific Inc. MA, USA) and image acquisition were driven by Nikon NIS Elements Imaging Software. ..

    Article Title: PCB Exposure in Adult Male Mice Reduces Proliferating Cells in the Prostate but Minimally Alters Voiding
    Article Snippet: .. An Eclipse Ci compound microscope fitted with a DS Ri2 camera (Nikon Instruments Inc., New York, NY, USA) with NIS elements imaging software (D 5.02.01) (Nikon Instruments Inc., New York, NY, USA) was used for imaging. ..

    Article Title: Codelivery of Doxorubicin and Carvacrol via a β-Cyclodextrin-Hyaluronic Acid Nanogel System for Targeted and Stimuli-Responsive Anticancer Therapy.
    Article Snippet: Nuclear staining was performed using PureBlu Hoechst 33342 Nuclear Staining Dye (Bio-Rad, Milan, Italy). .. Confocal laser scanning microscopy (CLSM) was performed using a C2 Plus confocal microscope (Nikon Instruments, Firenze, Italy), and images were processed with NIS-Elements Imaging Software (Nikon Instruments). ..

    Software:

    Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters
    Article Snippet: Live-cell imaging was conducted using a Nikon Ti-LAPP H-TIRF module with a CFI Apochromat TIRF 60 × oil objective (NA 1.49) or Apochromat TIRF 100 × oil objective (NA 1.49). .. Image acquisition was controlled by NIS-Elements Imaging Software (Nikon), with frames captured every ∼ 2 min. ..

    Article Title: PCB Exposure in Adult Male Mice Reduces Proliferating Cells in the Prostate but Minimally Alters Voiding
    Article Snippet: .. An Eclipse Ci compound microscope fitted with a DS Ri2 camera (Nikon Instruments Inc., New York, NY, USA) with NIS elements imaging software (D 5.02.01) (Nikon Instruments Inc., New York, NY, USA) was used for imaging. ..

    Article Title: Single molecule peptide sequencing
    Article Snippet: .. The Sutter Lambda 10-3 lter wheels (Sutter Instrument, CA, USA), motorized stage (Prior Scientific Inc. MA, USA) and image acquisition were driven by Nikon NIS Elements Imaging Software. ..

    Article Title: PCB Exposure in Adult Male Mice Reduces Proliferating Cells in the Prostate but Minimally Alters Voiding
    Article Snippet: .. An Eclipse Ci compound microscope fitted with a DS Ri2 camera (Nikon Instruments Inc., New York, NY, USA) with NIS elements imaging software (D 5.02.01) (Nikon Instruments Inc., New York, NY, USA) was used for imaging. ..

    Article Title: Codelivery of Doxorubicin and Carvacrol via a β-Cyclodextrin-Hyaluronic Acid Nanogel System for Targeted and Stimuli-Responsive Anticancer Therapy.
    Article Snippet: Nuclear staining was performed using PureBlu Hoechst 33342 Nuclear Staining Dye (Bio-Rad, Milan, Italy). .. Confocal laser scanning microscopy (CLSM) was performed using a C2 Plus confocal microscope (Nikon Instruments, Firenze, Italy), and images were processed with NIS-Elements Imaging Software (Nikon Instruments). ..

    Microscopy:

    Article Title: PCB Exposure in Adult Male Mice Reduces Proliferating Cells in the Prostate but Minimally Alters Voiding
    Article Snippet: .. An Eclipse Ci compound microscope fitted with a DS Ri2 camera (Nikon Instruments Inc., New York, NY, USA) with NIS elements imaging software (D 5.02.01) (Nikon Instruments Inc., New York, NY, USA) was used for imaging. ..

    Article Title: PCB Exposure in Adult Male Mice Reduces Proliferating Cells in the Prostate but Minimally Alters Voiding
    Article Snippet: .. An Eclipse Ci compound microscope fitted with a DS Ri2 camera (Nikon Instruments Inc., New York, NY, USA) with NIS elements imaging software (D 5.02.01) (Nikon Instruments Inc., New York, NY, USA) was used for imaging. ..

    Article Title: Codelivery of Doxorubicin and Carvacrol via a β-Cyclodextrin-Hyaluronic Acid Nanogel System for Targeted and Stimuli-Responsive Anticancer Therapy.
    Article Snippet: Nuclear staining was performed using PureBlu Hoechst 33342 Nuclear Staining Dye (Bio-Rad, Milan, Italy). .. Confocal laser scanning microscopy (CLSM) was performed using a C2 Plus confocal microscope (Nikon Instruments, Firenze, Italy), and images were processed with NIS-Elements Imaging Software (Nikon Instruments). ..

    Confocal Laser Scanning Microscopy:

    Article Title: Codelivery of Doxorubicin and Carvacrol via a β-Cyclodextrin-Hyaluronic Acid Nanogel System for Targeted and Stimuli-Responsive Anticancer Therapy.
    Article Snippet: Nuclear staining was performed using PureBlu Hoechst 33342 Nuclear Staining Dye (Bio-Rad, Milan, Italy). .. Confocal laser scanning microscopy (CLSM) was performed using a C2 Plus confocal microscope (Nikon Instruments, Firenze, Italy), and images were processed with NIS-Elements Imaging Software (Nikon Instruments). ..



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    Image Search Results


    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan).

    Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software